Certain chemicals are being used to control pests in agriculture field during insect pest management strategies. Quinalphos is one of them which are the extensively used organophosphorus (OP) insecticides and acaricides. World health organization (WHO) has assigned the status of “reasonably perilous” to this chemical; therefore, it is either forbidden or limited for usage in most of the countries. It is used against pests of wheat, rice, groundnut, cotton, sugarcane, coffee and other ornamental plants in India. Quinalphos is being broadly utilized by farmers in Andhra Pradesh, India. But, only 1% of the pesticide targets the pests, whereas the enduring 99% leaves harmful impacts on environment by contaminating soil, water and biota1.
It also inhibits of acetylcholinesterase (AChE) in non target idividuals2,3 as well undesirable effects on blood and brain functionality in chickens4 and fertility effectiveness in mature male rats5 have created an alarming situation in experts. Accordingly, natural resources including natural biota have gained the attention, regarding degradation of quinalphos in soil and water. In this regard, Venkata Subba and companion scientists conducted a research to isolate bacterial species having capability of degrading quinalphos in soil samples obtained from agricultural fields and to evaluate the extent of degradation by the biotic agent in liquid cultures following different circumstances. This investigation focuses to find a bioremediator for quinalphos contaminated environments.
In this experiment, scientists isolated a soil bacterium strain, able to use quinalphos as its solitary source of carbon and energy from soil by enrichment technique on a minimal salts medium (MSM). On the basis of morphological, biochemical and 16S rRNA gene sequence examination, it was found that the bacterium is closely closely associated to Bacillus subtilis and it is a species of the genus Bacillus. Quinalphos degrading abilities of this particular bacterium were analyzed under diverse culture circumstances. Then, researchers evaluated the Quinalphos degradation data through two-way ANOVA analysis with the Statistica v.10.
Venkata Subba and team discovered a Bacillus subtilis strain which has the capability to degrade the extremely poisonous pesticide active ingredient, quinalphos. The speed of degradation of quinalphos by B. subtilis was affected by temperature of incubation, pH, supplementary carbon and nitrogen source, inoculum compactness and amount of quinalphos. It was also revealed that 2-Hydroxyquinoxaline is a product of degradation of quinalphos; done by B. subtilis. Consequently, this discovered bacterium; B. subtilis can be suggested for eradicating the pollution of soil in result of quinalphos intoxification.
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23 August, 2019