Most of the plants are used for their medicinal properties. Herbal medicines are mainly accepted as antioxidants sources because of their phenol & flavonoid contents. In this aspect, researchers evaluated the possible biological targets of 3 plants with no known medicinal value but recognized for their region specific traditional use1.
The biological targets were enzymes of the antioxidant and xenobiotic resistance mechanisms, with task on inflammatory response. Detoxification and cellular antioxidant defense systems should be considered to analyze the possible biochemical screening function of extracts through corresponding concerned enzymes2.
The three plants used in the study were; Heliotropium europaeum, Carlina oligocephala and Echinops ritro. These used as herbal remedies in Eastern Europe in order to reduce inflammatory conditions3.
Out of 3 plants, first one wa used in soothing conditions such as warts, insect bites, inflammation of the joints and dose dependent hepatotoxic effects for humans, upon oral uptake4. Second plant used as a diuretic and stomach pain reliever, in addition to relieving inflammation symptoms of eczema, hemorrhoids and acne, also as a natural dye source for yellow coloring in Anatolia 5.
The third plant, roots are locally well-known to endorse lactation in nursing woman. It also pacify the breast swelling and pain and so known as anti-inflammatory for breast tissue. Some of these were found toxic for human consumption and livestock feeding, which was attributed to region specific levels of pyrrolizidine content and the method for extraction of plant6.
In the existing literature, the action mechanism by these plants is not clarified. Without any scientific evidence these are being used as traditional herbal remedies. Therefore, the extracts prepared from locally collected plants were evaluated for their antioxidant effects on cytosolic enzyme targets1.
For this purpose, Cytotoxic analysis were performed. The glutathione peroxidase (GPX), glutathione transferase (GST), catalase (CAT) and superoxide dismutase (SOD) targets, as well as their capacity to reduce free radicals (DPPH) and non-radical hydrogen peroxide (H2O2) were evaluated.
In the results it was found that best hydrogen peroxide scavenging and free radicals assays were obtained from the third plant. On other hand the best enzyme inhibition profile was observed with first plant against all biochemical targets.
It was conluded that the enzyme profiling of extracts may disclose the medicinal value of herbal remedies, by identifying their effects on cellular targets. In addition to define how reasonable the use of plants in traditional and complementary medicine (TCM) practices, these efforts may help to improve the standardized supplement preparations to benefit therapeutics with reduced efficiency due to inference with cellular defense and drug resistance enzymes, or both.
Written by: Rabeeia
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17 November, 2019