The genus Tribulus, consist of quite a few species which grow as shrubs or herbs in subtropical areas worldwide1. A research was conducted in order to evaluate the biological and photochemical properties of this plant. The study revealed potent and remarkable biological activities due to the presence of different active constituents2.
Tribulus terrestris L. is very familiar plant in Egypt. Commonly it is found in the Nile delta including Cairo. It is used in folk medicine for curing different diseases. The fruits and seeds are of great importance in folk medicine, they are used as diuretic, aphrodisiac, anthelmintic and to treat cough and kidney failure3.
In many countries it is used as a dietry supplement for sexual improvement, cardiac diseases and abdominal disorders4. Recently it was reported that the aqueous extract of T. terrestris L. acquires aphrodisiac properties because of androgen increasing properties1.
For determination of biological and phytochemical profile, different tests were conducted. Volatile volatile constituents were prepared and analyzed by GC/MS. Lipids were determined by gas liquid chromatography. The carbohydrate and the amino acid contents were prepared and analyzed by HPLC. Saponin fraction was prepared. The activities of polar, non-polar extracts and saponin fraction as aphrodisiac, antimicrobial, cytotoxic and antioxidant were evaluated.
It was found that volatile constituents showed to be 32 compounds, the major fatty acid in lipid test was found to be heptadecanoic acid. HPLC analysis showed the presence of insulin and glutamic acid. Saponin yield was 7.38%. The results of the biological investigation of the polar and non-polar extracts revealed their aphrodisiac, antimicrobial, cytotoxic and antioxidant activities with different percentages.
Phytochemical assessment showed the presence of phenolic and flavonoid contents. Separation and identification of rutin, diosgenin and quercetin were approved out using spectroscopic and chromatographic analysis.
All the acquired results corroborate the benefit of its use as effectual therapeutic agents from botanical origin. In the proper dosage from this can be realized after doing the necessary toxicological and pharmaceutical studies which are in progress.
Written by: Rabeeia
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18 August, 2019