Numerous physical and chemical methods have been employed up till now for the synthesis of AgNPs (Silver nano-particles)1. The main concern is use of harmful chemical agents used in AgNPs synthesis which accounts for same and green approaches for their manufacturing.
The biological approaches provide novel and safe ideas for nanoparticles synthesis as compared to traditional ones2. Use of fungi for biosynthesis of nanoparticles is advantageous as compared to plants, as fungi produce more protein which results in high production of nanoparticles3 and provides longer stability4.
Previously green synthesis of silver nanoparticles has been done using several fungal species as bio-agents. The major one includes; Trichoderma sp., Stachybotrys chartarum and Penicillium citrinum5, 6 etc.
Sweet pepper is one of important essential vegetable crop because of high nutritional value and antioxidant contents. Fusarium Wilt is major devastative and destructive fungal disease affecting crop production of pepper7. Therefore, improving the bio-production of this crop as well as vegetables is one of the objectives in agriculture in the world.
Engineered nanoparticles have achieved particular attention as a potential candidate for improving crop yield, resistance and disease management technologies. They can be used as fungicide as well as phyto-pathogens8. This led the scientists to perform study for biosynthesis of AgNPs using fungal biomass of Trichoderma harzianum and to evaluate the efficacy of AgNPs for suppression of plant pathogenic fungi in vitro9.
The AgNPs were synthesized and they were subjected to physical characterization using UV-Visible spectra, Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) images. Antifungal activity of synthesized AgNPs with poisoned food technique was used against phyto-pathogenic fungi.
Authors of the study conclusively said:
“It is possible to perform the biogenic synthesis of silver nanoparticles using T. harzianum as appropriate safe fungus. It should be mentioned that Trichoderma harzianum is not known to be harmful to humans. AgNPS at concentrations of 20, 40 and 80 ppm inhibited the F. oxysporum growth in vitro causing wilt of sweet pepper.”
Written by: Rabeeia
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17 November, 2019