Breast cancer (BC) is like a death alarm in women and mortality rates because of this deadly are accelerating ailment rapidly. According to a report, cases of female breast cancer will reach about 3.2 million per year by 20501. Genetic variations which lead to BC have been identified and accordingly; BRCA1/BRCA2 is the well known hereditary gene for BC. Yanwirasti, Wirsma A. Harahap and Dessy Arisanty conducted a research regarding BC and focused on microRNAs (miRNAs) which are small 22-25 nucleotides non-coding RNAs, which control gene expression primarily at post-transcriptional and transcriptional levels2.
miRNAs play a crucial role in regulation of metabolism3, cancer-related immune pathways and to arbitrate the secretion of immunosuppressive or immunostimulating4. Many miRNAs have been discovered which are significant for cell growth, such as differentiation, apoptosis, adhesion and other cellular processes3. It has been examined that abnormal expression of microRNAs leads towards cancer including breast cancer.
There are two types of miRNA; miRNA oncogenic (oncomiR) which inhibits the tumor suppressor gene and tumor suppressor miRNA (tumor suppressor miR) that inhibits the gene expression of oncogenes5. Both types may have the potential for miRNA gene therapies and alteration of miRNA levels is used diagnose cancer earlier and knowledge about progress of the disorder. Each miRNA gene has various targets at different phases of carcinogenesis6. On the other hand, miRNA levels and efficacy of treatment may reveal different outcomes in different patients. The miR-10b and miR-21 are an oncogenic miRNAs which play role in proliferation and invasion of Breast Cancer (BC) tumorigenesis. In the following investigation, scientists assessed that whether the expression of both miR-21 and miR-10b is different in BC tissue and (FATs ) Fibroadenomas (benign tumors) in the West Sumatra population.
During this research; 40 samples, consisting of 30 samples of BC tissues (control) and 10 samples of FATs tissues (non-cancerous ) were examined. Scientists evaluated the miR-21 and miR-10b expression of each sample by employing real time PCR, followed by universal Reverse Transcription (RT) then real-time PCR amplification with specific primers. Hsa-miR-16-5p LNA PCR primer was used as an endogenous control.
Yanwirasti and team concluded that for the sample of West Sumatran Women, Indonesia the expression level of miR-21 was 4 times higher in BC than in FATs. However, the expression level of miR-10b was lesser in BC as compared to FATs, by a factor of 3.34 fold. This investigation will help to diagnose and prevent the breast cancer prevalence globally.
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17 November, 2019