Scientists are relying on more natural resources to cope with yield and quality losses because of pests. Plant tissue culture is one of the most significant techniques to produced secondary metabolite against pathogens and degenerative diseases1. These diseases including cancer, diabetes and ageing are caused by reactive Oxygen Species (ROS). Accordingly, it is reported that natural compounds like terpenoids, phenols and alkaloids have potentials against degenerative diseases, particularly those caused by antioxidant activity2.
Fruits and vegetables such as dragon fruit, apple and tomato are abundantly found in Indonesia; which have treasure of natural compounds against degenerative disorders. For instance, Dragon fruit (Hylocereus polyrhizus) contains betacyanin pigments, polyphenols and flavonoids3, having anti-tumor, antioxidant and anti-inflammatory activity4-5. Antioxidant compounds commonly are capable to provide defense to human body cells and tissues from harmful impacts of free radicals. Moreover, apple (Malus sylvestris Mill.) also contains abundant amounts of antioxidant polyphenolic compounds, for various diseases caused by reactive oxygen and oxidative stress6. Additionally, tomato is a rich source of secondary metabolite such as lycopene to hinder free radical activity7.
Rumiyati and colleagues designed an experiment to search out the optimal explants from dragon fruit, apple and tomato which can produce effective culture medium to support calluses growth to induce regeneration and to evaluate the optimal culture medium for its growth. Callus is a mass of irregular, unrecognized cells; having ability to produce metabolites like the parent plant8. In addition, the callus cells also have totipotency and plasticity properties, which help to alter their metabolism and to adjust their growth according to environment9.
In this research; scientists assessed the optimal ratio of sucrose and 2,4-D in Murashige and Skoog (MS) medium for callus induction from various plant organ explants to develop natural cell regeneration agent by employing analytical technique. Explants were developed in different concentrations of combination between MS medium and 2,4-D. Afterwards, callus growth, including their weight and surface was calculated and analyzed through one way analysis of variance (ANOVA).
Researchers noted the callus growth from its explants within 5-7 days after induction. The maximum value of fresh weight of dragon fruit callus was obtained through MS supplemented with 1 μL L–1 2,4-D and 30 g sucrose. On the other hand, apple and tomato callus induction and growth maintenance exhibited optimal performance by MS supplemented with 30 g sucrose and 2 μL L–1 2,4-D. This study will enhance the scope to develop natural cell regeneration agent to encourage revival upon cell damage.
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17 November, 2019