Scientists have establish biofabrication of CuO NPs using strawberry tree (Arbutus unedo) leaf extracts and examined the in vitro cytotoxicity against nasopharynx cancer cell lines (KB cells). The CuO NPs showed their biocompatibility nature towards KB cells1.
Copper oxide nanoparticles (CuO NPs) have vast range of applications in nanotechnology. They are being used in textiles, wall paints, plastic materials for killing microbes and as well as in production of pesticides2, 3.
CuO NPs are developed through various synthetic approaches including; microwave reactions, chemical reduction and electro-chemical methods etc. using chemical methods for manufacturing CuO NPs makes them unsafe to use for biological studies because of absorption of certain toxic chemicals on their surface. So an eco-friendly method of CuO NPs production is required4.
The biosynthesis of silver or gold nanoparticles by using different plant and bacterial extracts has already reported in literature5, 6. Many synthetic reports have also been established via synthetic approach. This lead scientist to synthesize them, using Arbutus unedo leaf extract and checked the cytoxicity against nasopharynx carcinoma cells 1.
Researchers used strawberry tree leaf extracts and mix them with aqueous solution of copper sulfate. Brown color solution was obtained after 10 minute of stirring. The prepared CuO NPs have been studied by XRD, FT-IR, UV–Vis, EDS and TEM characterization techniques. The in vitro cytotoxicity against KB cells was determined using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay.
Transmission electron microscopy revealed the formation of spherical CuO NPs. The crystalline nature of the synthesized NPs was confirmed by XRD measurements of dried particles. The in-vitro cytotoxicity of Arbutus unedo extract mediated CuO NPs against nasopharynx cancer cell lines (KB cells) have confirmed their biocompatible nature.
In this way a low cost, easy and environment friendly approach for biofabrication of copper oxide nanoparticles using strawberry tree extracts. The FTIR analysis revealed the existence of biomolecules present in Arbutus unedo extract onto the CuO NPs surface.
Further, TEM and DLS analysis confirmed the formation of spherical CuO NPs with average size of 30 nm. Additionally, Arbutus unedo extract mediated CuO NPs have exhibited good biocompatibility, making them useful as cell labeling agents.
Written by: Rabeeia
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17 November, 2019