Reactive Oxygen Species (ROS) or excessive production of oxidants and sometimes pathologic circumstances cause accumulation of free radicals in the body which can leads towards oxidative stress1. It is reported that this condition may cause ageing2 and other linked ailments including cancer3, cardiovascular diseases4, Alzheimer’s disease5 as well as Parkinson’s disease6 etc.
To combat with this situation, body produces antioxidants and accordingly, Polyphenolic compounds and flavonoids are considered as potent antioxidants. According to Kumar and team7, Crotalaria verrucosa consists of flavonoids, steroids as well as steroidal nucleus. C. verrucosa, is basically a perennial shrub which is available in several regions of Bangladesh and it is the only member of Crotalaria species which possesses medicinal value8. It is considered as effectual chiefly agaisnt impetigo, scabies, salivation, jaundice, cough, biliousness, dyspepsia, fever, cardiac abnormalities and oral diseases9. Chakma and Marma tribes of Bangladesh also use this as a medicinal plant for the treatment of skin allergies. However, investigations regarding evaluation of the antioxidant and cytotoxic potential of C. verrucosa methanolic leaf extract (CVMLE) have not been conducted yet.
Hence, Zainab Syed Ahmed10 and her research team decided to carry out a study to examine the antioxidant and cytotoxic potential of C. verrucosa to identify and quantify those metabolites that perform these particular activities. In this experiment, cold maceration by utilizing methanol was employed for extraction from C. verrucosa leaves. Scientists then assessed the antioxidant activity via total phenol content, total flavonoid content, total antioxidant capacity as well as DPPH free radical scavenging assays and metabolite profiling was obtained through HPLC-DAD. At the end, research team determined the cytotoxicity by means of MTT assay against HeLa cells.
Conclusively, scientists noted a strong cytotoxic potential and a moderate antioxidant potential of C. verrucosa which confirms its function in folkloric remedies but extract requires more purification to employ it as a folkloric remedy for oxidative stress-mediated ailments. Therefore, these outcomes can help to study in vivo antioxidant and in vivo cytotoxic activity to examine the bioactivity potential of this medicinal plant in a better way.
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24 April, 2019