Glioblastoma multiforme commonly known as Glioma is most frequently diagnosed brain tumor found in adults. It has very high rate of death and morbidity1. It is one of most malignant tumor having high reoccurrence rate with 2 years of survival period2. The common cause of patient’s death with glioma is not the presence of tumor but the tumor metastasis rate3.
The promising glioma treatment includes, inhibition of tumor metastasis is necessary at tumor spot as well as in adjacent organs. Many studies have been conducted to screen the molecules for their role in the inhibition of carcinoma cell proliferation, migration and invasion.
Gedunin is phytochemical isolate of the Indian neem tree having vast range of biological activities such as; anticancer, antimalarial and insecticidal4. Previously it has been demonstrated that Gedunin treatment imparts anti-proliferative effect through alteration in the expression of heat shock protein 90 kDa (Hsp90)5.
Together with both information and evidences scientists decided to investigate the effect gedunin on inhibition of cell proliferation, migration and invasive potential of U-251 MG glioma cells. Through this experiment it was observed that gedunin treatment significantly inhibited the proliferation, incursion and drifting potential of U-251 MG cells6.
For this purpose, cell culture was prepared for U-251 MG cells followed by cell viability, invasion and migration assays. Western blotting was done followed by real time PCR, and total mRNA was calculated.
From the experiment it was revealed that gedunin displayed a time and concentration dependent inhibitory effects on the U-251 MG cells’ viability. It was also observed that gedunin treatment inhibited the migration of U-251 MG cells. Lower invasive tendency and significant reduction in many expression proteins was also found.
From all above observations, scientists conclude that gedunin treatment exhibits inhibitory effect on the proliferation, migration and invasive potential of the glioma cells. Therefore, gedunin can be of the therapeutic importance for the treatment of glioma.
Written by: Rabeeia
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23 August, 2019