Dental caries is one the leading oral health ailments. It is also termed as tooth decay in which progressive devastation of the infected teeth occurs because of chronic infection; caused by acid-producing bacteria 1. 60-90% of schoolchildren and about 100% of adults have suffered with tooth decay worldwide; according to reports of WHO2. Dominant pathogenic species causing dental caries is Streptococcus mutans, (the facultative anaerobic gram-positive bacteria)3,4.mutans leads towards tooth surface adherence, biofilm or dental plaque formation and acid production (acidogenesis)4. For that reason, suppression of cariogenic outcomes is necessary in addition to an inhibition against S. mutans growth, for the prevention and cure of dental caries. Dentists are shifting towards natural control of tooth decay because of adverse side impacts of chemicals, being used to control this disease.
Derris reticulata (Cha-em-nuea in Thai) is a medicinal plant; found in almost every region of Thailand. It is being used in Thai folk medication to cure productive cough and throat diseases as well as 5. The major phytochemical constituents; prenylated flavanones including lupinifolin, 2’’’,3’’’-epoxy lupinifolin, dereticulatin and 1’’’-hydroxy-2’’’,3’’’-epoxylupinifolin were reported in D. reticulata stem 6,7. Lupinifolin exhibited antibacterial activity against various Gram-positive bacteria particularly Staphylococcus aureus with the MIC (minimum inhibitory concentration)and MBC of 8 and 16 μg mL–1, respectively8.
Pawitra Pulbutr and team designed an experiment to evaluate the antibacterial and anticariogenic properties of the D. reticulata ethanolic stem extract. To authenticate the D. reticulata sample, scientists performed TLC (Thin Layer Chromatography) analysis. Minimum inhibition concentration and minimum bactericidal concentration were calculated by using broth dilution and drop plate techniques, respectively. Sucrose dependent and sucrose independent-adherences, biofilm formation and glycolytic pH drop assays were performed to determine the anticariogenic activity.
Researchers noted antibacterial activity of D. reticulata against S. mutans with the MIC and MBC of 0.875±0.250 and 1.750±0.500 mg mL–1, respectively. The extract at the lower concentrations of sub-MIC also showed maximum inhibition against the cariogenic properties of S. mutans, including surface adherence, biofilm formation and glycolytic acid production. Scientists concluded that D. reticulata stem extract has a considerable anticariogenic attributes and it can be proved as potential oral health care product for dental caries treatment in future.
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22 August, 2019