Tamoxifen is an anticancer compound. Its concentration is measured in plasma levels. In a new study scientists have developed a new in order to find its concentration in plasma. It was found that the proposed method concurrently measured the concentrations of tamoxifen and 4-OHTAM in plasma in female rats1.
Tamoxifen is a non-steroidal anti-oestrogen receptor modulator (SERM) used in the treatment and prevention of breast cancer2. It has been accounted that continuing use of tamoxifen raises the risk of endometrial cancer linked with the increased formation of 4-OHTAM (4-Hydroxytamoxifen, a metabolite of tamoxifen) 3.
The things of anti-oestrogen and the harmful effects of tamoxifen and its metabolited depend on their levels in plasma. The plasma levels of these were found to get affected by ethyle acetate fraction of Myrmecodia plant. In the study researchers wanted to develop a selective, sensistive and valid procedure in order to quantitatively determine the concentration of tamoxifen and 4-OHTAM in plasma1.
For this purpose Tamoxifen and its metabolite were extracted from plasma by protein precipitation. It was analyzed using liquid chromatography. 30 female white rats were used for this study. They were then divided into 6 groups.
Numerous methods have already been reported for analysis of tamoxifen and its metabolite, 4-OHTAM4. But none of them can be directly applied in lab. Each lab has its own explicit condition which is specified for them. This new developed method resulted in faster retention times and easier sample preparation and can be injected in chromatography.
The scheme anticipated in the current study has several advantages over previously published methods reported in the literature for tamoxifen, such as simple sample preparation procedures using protein precipitation, a short analysis time (5 min per sample) and high sensitivity. Moreover, the method was used to measure the concentration-time profiles of tamoxifen and 4-OHTAM for a toxicology/carcinogenesis study of rat plasma.
So it was concluded that it also met the 2011 EMA bioanalytical guidelines for validations with concentrations ranging from 2.0-200.0 ng mL–1.
Written by: Rabeeia
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18 August, 2019