Panax ginseng or Ginseng is a native herb to East Asia, China and Southeast Asia. It has been used as herbal medicine for thousands of years. It contains many pharmacological properties1. Ginseng extract contains several ginsenosides; Rb1, Rb2, Rc, Rd, Re, Rf and Rg1 and these ginsenosides are believed to be involved in the pharmacological activity of the ginseng2.
However, insufficient quantity of ginsenosides in ginseng extract has delayed their employment for treatment purpose. Therefore, the advancement of ginseng extract containing an increased concentration of ginsenosides is of great interest.
The gene rolC is derived from bacteria Agrobacterium rhizogenes. It plays an essential role in the hairy root disease development and its expression in plants can cause large morphological and biochemical variations3.
Recently the function of rolC gene was found in formation of shoot meristems, indicating that it might play a critical role in pluripotent stem cells formation4. It was also reported that this gene could be amplified and cloned via PCR5.
Since the role of the rolC gene in hairy roots, it was hypothesized that relationship could be existed between the rolC gene level and ginsenosides concentration. Researchers investigated the role of rolC gene on ginsenosides content in ginseng hairy roots in order to improve the ginsenosides content in ginseng 6.
Genomic DNA was extracted from ginseng hairy root. The rolC gene was cloned in recombinant expression plasmid. The mRNA and protein of rolC gene were then detected and the different types of ginsenosides were evaluated. Ultimately the effects of rolC gene infection on the ginsenosides content were then evaluated.
The results showed that rolC gene introduction could result in increased ginsenosides content in ginseng hairy roots and 4 weeks was confirmed as the optimal time for ginseng collection. It was established that introduction of rolC gene could promote ginsenosides content in ginseng hairy roots, which would be beneficial for obtaining ginseng extract containing high concentration of ginsenosides
Written by: Rabeeia
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18 August, 2019