Medicinal value of different plant species is crucial and trend to use organic products is being accelerated rapidly. Cressa Linn. and Cuscuta Yunck are playing vital role in this regard. The genus Cressa is perennial herbaceous plant1 which is being used in herbal medication as tonic, stomachic, anthelmintic and to cure hepatic disorders, urinary discharges, diabetes, asthma, ulcers, constipation and also in aphrodisiac purposes2. While, the genus Cuscuta is prevalent parasitic weed, which have been utilized in China for medicinal purpose for many years; to cure fresh wounds, hepatic, stomach and urinary tract disorders3.
An experiment was performed to evaluate the association between these two genera samples related to the same family Convolvulaceae. Three molecular techniques (PCR based DNA fingerprint), RAPD (5 primers), ISSR (6 primers) and SCoT (8 primers) were used to assess their molecular basis. Deoxyribonucleic acid (DNA) fingerprinting is popularly known for genetic identification in plant breeding and germplasm management of cultivar or varietal recognition4 and to identify the links between different plant genera in the same family. While, Random amplified polymorphic DNA (RAPDs) are DNA fragments amplified through PCR by means of short synthetic primers (generally 10 bp) of random sequence. These oligonucleotides serve as both forward and reverse primer, and are generally able to magnify fragments from 1-10 genomic sites simultaneously.
Simple Sequence Repeat (ISSR) amplification is a different method, which makes the usage of anchored primers to amplify simple sequence repeats without the requirement for prior sequence information5. This technique is more reliable than the RAPD technique. On the other hand, start codon targeted (SCoT) polymorphism is suggested as a new, simple and reliable gene-targeted marker technique based on the translation start codon6.
Results showed that implementing RAPD technique discovered that using OP-A02, OP-A09, OP-A10, OP-C04 and OP-M01 primers recorded 60, 83.33, 100, 50 and 70.66% polymorphism, respectively. While, ISSR technique recorded 40, 50, 100, 66.67, 33.33 and 37.5% polymorphism with 44B, HB-08, HB-09, HB-10, HB-11 and HB-12 primers, respectively. Moreover, amplification of SCoT technique, SCoT 1, SCoT 2, SCoT 3, SCoT 4, SCoT 6, SCoT 8, SCoT 10 and SCoT 12 primers recorded 33.33, 28.57, 14.28, 66.66, 25, 40, 42.85 and 50%, respectively . The total polymorphism recorded 73.33, 54.58 and 37.7% for RAPD, ISSR and SCoT techniques, correspondingly. Conclusively, this research exhibited that SCoT technique is more effective and sustainable to distinguish two genera under observation, Cressa Linn. and Cuscuta Yunck as compared to ISSR and RAPD techniques.
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23 August, 2019