Worth of medicinal plants can’t be denied because of accessibility, reasonable costs and zero harmful impacts therefore; people are tending towards more natural resources. Herbal plants are significant source of herbal products which produce certain chemical substances having medicinal value. Accordingly, phenolic compounds including flavonoids, alkaloids and tannins are the most vital compounds of the medicinal plants1. Gardenia jasminoides is one of them and used as hedges, borders, screens, borders or ground covers. Moreover, it is plays chief role in the traditional Chinese medicine and is being used to treat muscles injuries, tendons and joints2. Gardenia jasminoides is also a rich source of phenolic compounds and anti-inflammatory flavonoids. Therefore; this plant can be used against inflammatory diseases and also for pain treatment3.
The major components of Gardenia’s fruits are iridoid glycosides, which are derivatives of crocetin with coloring characteristics and meticulous water soluble behavior, opposing most plant families of carotenoid. Carotenoids serve as the antioxidant content of plants and have pharmacological properties, such as preventing cardiovascular diseases4. The TDZ and zeatin are considered as cytokinins that promote cell division. There are two types of cytokinins, adenine type like zeatin and phenyl-urea type like TDZ5.
In this regard; Ahmed Mohamed Magdy Gabr and colleagues designed an experiment, to recognize the effect of zeatin and thidiazuron (TDZ) on callus proliferation, total phenolic content, total flavonoids and DPPH scavenging activity of gardenia callus cultures. Scientists cultured the calli on Murashige and Skoog (MS) medium supplement with various concentrations (2, 4 or 6 mg L–1) of zeatin or TDZ individually as well as combination of 2 mg L–1 zeatin+4 mg L–1 TDZ. Cultures contained 4 mg L–1 TDZ exhibited maximum callus fresh weight followed by those contained 2 mg L–1 zeatin then that cultured on 4 mg L–1 zeatin. Data reported as Mean±Standard Deviation (SD). Data were subjected to one-way analysis of variance (p< 0.05) and results were processed by Excel (2010) and SPSS Version 17.0.
Ahmed and team noted that the HPLC analysis for phenolic acids exhibited highest contents of chlorogenic acid, rosmarinic acid and cinnamic with callus cultured on 4 mg L–1 TDZ (123.24, 322.14 and 278.22 μg g–1, respectively). Through HPLC analysis, rutin, apigenin-7-glucoside and kaempferol were detected regarding flavonoids. On the other hand, apigenin-7-glucoside was detected with elevated content (129.86 μg g–1) in callus culture with 4 mg L–1 Zeatin. Therefore, scientists concluded that the HPLC analysis recommended that TDZ is more effectual in accumulation of individual phenolic and flavonoid than Zeatin. Moreover, these findings provide a constructive system for further investigation on in vitro culture of G. jasminoides as alternative and novel source for imperative secondary products.
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17 November, 2019