The Pesisir cattle breed is one of the native cattle breeds in Indonesia that has been acclimatized to unfavorable conditions, particularly to hot and humid climate and inferior feed quality to produce meat. It costs less as compared to other breeds therefore; its customers are accelerating. Currently, selection for better performance of this precious breed has attained focus of experts, chiefly with the progression of genetically molecular biotechnology1. Accordingly, insulin-like growth factor 1 (IGF1) or somatomedin gene is crucial gene in a range of physiological and metabolic procedures. It is the mediator of various biological effects, e.g. it accelerates the absorption of glucose, stimulates myogenesis, inhibits apoptosis, participates in the activation of cell cycle genes, amplifies the synthesis of lipids, stimulates the production of progesterone in granular cells and intervenes in the synthesis of DNA, protein, RNA and in cell proliferation2.
Two single nucleotide polymorphism of IGF1 gene namely IGF1/SnaBI and IGF1/TasI are linked with production characteristics in several cattle breeds. It was reported that in Angus cattle, the SNP IGF1/SnaBI was observed to be a effectual molecular marker linked with weight gain during the first 20 days after weaning3. In 2011, Szewczuk and colleagues4 discovered an important connection of (p<0.05) of IGF1/TasI polymorphism with milk production, fat and protein yield where in the 2nd and 3rd lactations where cows carrying the CC genotype produced more milk than the AA individuals. Moreover, one single nucleotide polymorphism in exon 4 namely IGF1/RsaI has a noteworthy effect (p<0.01) on birth weight, weaning weight and average daily gain of Bali cattle5. In the experiment, performed by Yurnalis and colleagues the isolation of DNA from 183 blood sample was conducted by employing DNA extraction kit (Promega DNA purification kit) following the manufacture process. The amplification of IGF1 fragment was done by master mix from Thermo Scientific. The purpose of this research was to evaluate the IGF1/SnaBI, IGF1/TasI and IGF1/RsaI polymorphisms and their interlinkage with average daily gain in Pesisir cattle.
Scientists noted three genotypes in the case of the IGF1/SnaBI polymorphism and frequencies were 0.011, 0.011 and 0.978 for AA, AB and BB, respectively, which showed the frequencies: 0.064 and 0.9836 for A and B alleles. IGF1-TasI locus exhibited that AA genotype present with a frequency of 0.978, AC with 0.011 and CC with 0.011 with frequency for allele A and C were 0.9836 and 0.0164, respectively. In the case IGF1/RsaI polymorphism, only TT and CT genotype with frequency 0.027 and 0.973 were produced respectively and frequency of T and C allele was found as 0.5137 and 0.4863, respectively. Researchers concluded that among three polymorphisms; only IGF1/RsaI is interlinked with average daily gain. Therefore, this polymorphism can pave a way to improve body weight in Pesisir cattle.
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21 August, 2019