Cryptosporidium is a protozoan parasite which causes cryptosporidiosis, by invading intestinal epithelial cells (IECs) to complete its life cycle1. Despite of the genetic investigations on this parasite, its signal peptide proteins is still unrevealed which is main challenge for experts. Up till now, only one research is performed regarding signal peptide linked with filarial nematode parasite 2.
With the revolution in molecular genetics many new procedure are being introduced in this regard and SignalP version 4.1 is found to be the most precise program to examine protein sequence at N termini to evaluate co-translational as well as translocation of proteins3. During manufacturing of drug against Cryptosporidium, it was noted that ES proteins is crucial due to its function of parasitism in cryptosporidiosis4.
Some researchers investigated Excretory-secretory (ES) products to discover the potential signal peptides from Cryptosporidium proteins. Scientists stated that these proteins might inherit from independent progenitors to be utilized for parasitic adaptation5,6. Moreover, they possess a key role in parasitism as well as modulation of host immunity7.
In this regard, Mohd Aiman, Muhammad Lokman and Afzan Mat8 investigated selected 100 putative protein sequences of Cryptosporidium parvum randomly in its established genome database for signal peptide. For this purpose, scientists performed Prediction analysis of protein sequences and identification of signal peptides on this parasite were examined through SignalP 4.1 server. Out of 100 tested protein sequences, 6 (6%) were found to be predicted as signal peptide sequences.
On the basis of the secretary proteins in Cryptosporidium, many virulence protein sequences of this parasite probably exhibited the existence of N-terminal signal peptide or not. Therefore, detailed investigation is needed to identify N-terminal signal peptide function in Cryptosporidium to assist its virulence and pathogenesis pathway.
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17 November, 2019