Jojoba (Simmondsia chinensis) is one of the most versatile plants because of its acclimatization towards hot and dry climates. Jojoba oil is being utilized in cosmetics as well as pharmaceutical sector. Jojoba is a dioecious species (having the male and female reproductive organs in separate plants). Descendants of jojoba are extremely because it is a cross pollinated crop, therefore due to diversity in seedlings; economic viability of jojoba has become uncertain. However, direct selection of high quality genotypes and their evaluation after vegetative propagation has led towards more improved crops1.
Micro propagation through biotechnological techniques produces elite and true to type plants2. Moreover, it is reported that somatic embryogenesis in jojoba can be done from zygotic embryo3,4 and leaf explants5. Conversely, propagation of callus cultures is significant because jojoba oil can be e from a extracted through variety of explants including in vitro callus cultures6, which can reduce the reliance on seeds for oil production7. The callus culture medium is supplemented with cytokinins such as TDZ and zeatin, which initiates callus formation or somentic embryogenisis. A lot of physiological and biochemical processes in cells are improved through TDZ, but, its mode of action is still unidentified8.
Seeds which consist of zygotic embryo as well as somatic embryos are the major source of secondary metabolites. Accordingly, Amal Abd El-Latif El-Ashry and colleagues performed an experiment to stimulate embryogenic calli formation from jojoba by utilizing zeatin and thidiazuron (TDZ), and evaluation of the antioxidant activity of proliferated calli. In this experiment, leaf and stem explants obtained from in vitro grown jojoba shootlets, were cultured for callus induction on Murashige and Skoog (MS) medium with dissimilar mixtures of 0.5 mg L–1 benzyl adenine (BA) or kinetin with 2,4-Dichlorophenoxyacetic acid (2,4-D), Naphthalene acetic acid (NAA) and picloram at 0.5 or 1mg L–1. Friable callus were shifted to woody plant medium (WPM) having various concentrations of zeatin or TDZ; to trigger calli formation. Afterwards, scientists assessed the antioxidant activity of different treatments by employing hexane or petroleum ether extraction. Moreover, data was examined as mean±standard deviation (SD).
Scientists concluded that zeatin containing treatments were more effectual to promote callus formation, by using callus from leaves of explants. Whereas, treatments having TDZ exhibited more efficacy in inducing formation of embryogenic callus through stem explants. Moreover, it was that also noted that DPPH %age in callus obtained from leaves was elevated as compared to callus gained from stem by utilizing hexane as extraction solvent with all treatments. The embryogenic calli from leaves originated on 2 mg L–1 zeatin exhibited satisfactory potential with hexane extraction. These findings will sharpen the horizon and paver a way towards efficient propagation techniques of jojoba plant.
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17 November, 2019