Phytochemicals are significant chemical compounds produced by plants, which usually help them to defend against predators and pathogens. It is reported that techniques like plant tissue culture methods are crucial to produce worthy phytochemicals. Moreover, plant tissue methods leads to efficient production of secondary metabolites1. Accordingly, scientists found that in vitro cultures consist of elevated concentrations of secondary metabolites as compared to original plant2,3.
Flavonoid compounds are one of the most momentous phytochemicals found in many plants including Keppel fruit plant (Stelechocarpus burahol). These flavonoids are effective antihyperuricemic for gout medication. Accordingly, Purwantiningsih and team4 performed a research and the outcomes of in vivo tests on both the ethanol extract and hexane extract of S. burahol exhibited their efficacy in lowering blood uric acid levels. The S. burahol also have antioxidants in its bark, flowers and fruit5. One of the flavonoid compounds in S. burahol is 3, 7, 3’, 4’ tetrahydroxy 5 methyl flavone, which is the most active antioxidant6.
Cell cultures used in plant tissue culture techniques are composed of homogeneous and among them some cells undergo differentiation e.g. Cupressus macrocarpa showed oblong cells in the 2nd week of culture and then transformed into ellipse shape in the next 2 weeks7. On the other hand, C. arizonica cell suspension culture constantly maintained globular shaped cells till the end of the growth period7. Noor Aini Habibah and team performed an experiment to evaluate flavonoid production, growth and cell differentiation of S. burahol in cell suspension culture. In this experiment scientists planted the mesocarp in Murashige and Skoog (MS) medium supplemented with 7.5 mg L–1 picloram for the induction of callus to obtain Non-embryonic callus which then utilized in the development of cell suspension cultures. Growth of cells was assessed by fresh and dry weights. Noor and colleagues also evaluated the fresh weight, dry weight and flavonoid content as a result of culture status.
It was noted that the growth of the S. burahol cell suspension was dawdling and the stationary phase happened at 30 days. Moreover, the highest production occurred on the 15th day of the log phase as the production of flavonoids was not parallel with the growth of cells. The globular-shaped cells dominated the cell suspension culture at all ages. Fluorescein diacetate (FDA) staining of cells derived from cell cultures aged for 36 days exhibited that some cells were still viable. Conclusively, the trial showed that flavonoid production, growth and cell differentiation of a S. burahol cell suspension culture varied according to the culture age. This investigation will ultimately sharpen the horizons regarding phytochemicals and their potential against pests in agriculture.
Your email address will not be published. Required fields are marked *
23 August, 2019