With the advancement in technology, scientists are introducing novel methods to diagnose various diseases. Accordingly, some of these techniques are as follows. Flotation process is a a useful way to yield obvious preparation because it separates protozoan cysts, coccidian oocysts and certain helminths’ eggs and larvae from excess debris by using solution with a high specific gravity. Whereas, another procedure known as sedimentation procedure doesn’t provide clear results but, some helminths’ eggs (which cannot be uncovered through flotation method) can be exposed with sedimentation1,2.
Formalin ether concentration technique (FECT) is a different way to diagnose helminths as well as intestinal protozoa3,4. But this is risky as it carries fire and explosion hazards. Moreover, few parasitic elements can also be misdiagnosed.
Recently, experts have developed the Mini-FLOTAC from FLOTAC (a novel apparatus used to count multivalent faecal egg) with a plus point that it doesn’t need centrifugation. This techinique can be used for both diagnostic as well as epidemiological purposes within limited sources. Moreover, it has the replace ability to substitute FLOTAC combining sensitivity as well as low costs5,6.
In this regard, Rania Mohammad7 and colleagues designed an experiment to evaluate the actual effectiveness of Mini-FLOTAC against other copro-parasitological methods, wet smear and sedimentation as well as flotation concentration for diagnosis of intestinal parasitic infections. For this purpose scientists performed three investigations. The 1st experiment was employed to examine the performance and sensitivity. While, the 2nd was used to assess the percent accuracy, precision as well as sensitivity and the 3rd; a field study, for validating its sensitivity and predictive value.
At the end of this research, Mini-FLOTAC proved significant sensitivity in analysis of helminths as Mini-FLOTAC (FS3) technique exhibited the best outcomes, to diagnose H. nana and E. vermicularis. However, further investigation is required to evaluate its ability to identify protozoa.
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24 August, 2019