Sulforaphane is the organosulfer has is found in cruciferous vegetables like cabbage and broccoli. This compound inhibits the expression a specific gene which causes various inflammatory diseases, a new study predicted1.
The appearance of secretory group IIA phospholipase A2 (sPLA2-IIA) has been shown to be eminent in various inflammatory diseases and Lipopolysaccharide (LPS) up-regulates the expression of sPLA2-IIA in HUVECs i.e. Human umbilical vein endothelial cells2.
In a previous reported it was reported that sulforaphane possesses anti-inflammatory properties, resulting in the down regulation of LPS-stimulated inducible (iNOS) Nitric Oxide Synthase3. But there is no previous study which has showed the effects of sulforaphane on the expression and activity levels of sPLA2-IIA.
Scientists firstly hypothesized that sulforaphane will reduce the expression and activity levels of sPLA2-IIA. Scientist took an investigation for this and also deteremined it’s potential as a useful drug candidate in the treatment of inflammatory diseases1.
In order to achieve the desired results, cells were treated with sulforaphane once the HUVECs were activated with LPS. Cecal ligation and puncture (CLP) or LPS-injected operated mice were administrated SFN in vivo. After that the effects of sulforaphne in expression and activity of sPLA2-IIA were determined by ELISA. The cytostolic and extracellular activities were also monitored.
It was found that the by post treating cells or mice with sulforaphane the inhibition of LPS- or CLP was induced in expression and activity of sPLA2-IIA. It was also found that sulforapahne suppressed the activation of cytostolic and extracellular activities by LPS.
Written by: Rabeeia
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17 November, 2019